Summary
Type: SPE
Application #: 305520
Catalog#: 730936 - SPE Cartridges, Chromabond, HR-X, 3ml, PP, 60mg, 30/pk
Detection:
Conditions: Column conditioning: 5 mL methanol, then 5 mL ultrapure water
Sample aspiration: 50 mL water sample is passed through the column by vacuum
Washing: 5 mL ultrapure water with 5 % methanol
Drying: with gentle nitrogen stream, 15 min
(storage before elution step at 4 °C is possible for 1 month)
Elution: 2 x 3 mL methanol; then 1 x 3 mL methanol – ethyl acetate (75:25, v/v)
Evaporation: after addition of 10 µL internal standard the mixture is evaporated to dryness
Reconstitution: in 1 mL of the initial HPLC eluent
Authors: Lissalde, S. et al.
Source: J. Chromatogr. A 1218 (2011), 1492–1502